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验证数据展示

  • WB analysis using 15319-1-AP

    WB analysis using 15319-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15319-1-AP (AP3S2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15319-1-AP

    IHC staining of human colon cancer using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15319-1-AP

    IHC staining of human colon cancer using 15319-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15319-1-AP (AP3S2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AP3S2 Polyclonal antibody

AP3S2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15319-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AP 3 complex subunit sigma 2, AP 3 complex subunit sigma 3B, AP3S2, AP3S3, Sigma 3B adaptin, Sigma adaptin 3b, sigma3b, Sigma3B adaptin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, human testis tissue, mouse ovary tissue, mouse testis tissue, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15319-1-AP targets AP3S2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AP3S2 fusion protein Ag7565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 3, sigma 2 subunit
别名 AP 3 complex subunit sigma 2, AP 3 complex subunit sigma 3B, AP3S2, AP3S3, Sigma 3B adaptin, Sigma adaptin 3b, sigma3b, Sigma3B adaptin
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC002785
基因名称 AP3S2
Gene ID (NCBI) 10239
RRIDAB_2056644
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP59780
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP3S2 is a 22-kDa protein that belongs to the adaptor complexes small subunit family. AP3S2 is a subunit of the AP-3 complex which is associated with the Golgi region as well as more peripheral structures. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-3 complex is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2).

实验方案

Product Specific Protocols
WB protocol for AP3S2 antibody 15319-1-APDownload protocol
IHC protocol for AP3S2 antibody 15319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols