验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, HeLa cells, mouse lung tissue, A431 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11653-1-AP targets AP4M1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | AP4M1 fusion protein Ag2222 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | adaptor-related protein complex 4, mu 1 subunit |
| 别名 | AP 4 complex subunit mu 1, AP4M1, MU 4, Mu adaptin related protein 2, MU ARP2, Mu subunit of AP 4, mu4, Mu4 adaptin, MUARP2 |
| 计算分子量 | 453 aa, 50 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC018705 |
| 基因名称 | AP4M1 |
| Gene ID (NCBI) | 9179 |
| RRID | AB_2227267 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00189 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4M1 is a member of the AP4 complex, which also includes AP4B1, AP4E1, and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4M1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AP4M1 antibody 11653-1-AP | Download protocol |
| IHC protocol for AP4M1 antibody 11653-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
