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AP4M1 Polyclonal antibody

AP4M1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11653-1-AP

Print datasheet

Synonyms

AP 4 complex subunit mu 1, AP4M1, MU 4, Mu adaptin related protein 2, MU ARP2, Mu subunit of AP 4, mu4, Mu4 adaptin, MUARP2

验证数据展示

  • WB analysis of mouse heart using 11653-1-AP

    WB analysis of mouse heart using 11653-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11653-1-AP

    IHC staining of human lymphoma using 11653-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11653-1-AP (AP4M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 11653-1-AP

    IHC staining of human lymphoma using 11653-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11653-1-AP (AP4M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, mouse lung tissue, A431 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11653-1-AP targets AP4M1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AP4M1 fusion protein Ag2222 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 4, mu 1 subunit
Synonyms AP 4 complex subunit mu 1, AP4M1, MU 4, Mu adaptin related protein 2, MU ARP2, Mu subunit of AP 4, mu4, Mu4 adaptin, MUARP2
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC018705
Gene Symbol AP4M1
Gene ID (NCBI) 9179
RRIDAB_2227267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00189
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4M1 is a member of the AP4 complex, which also includes AP4B1, AP4E1, and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4M1.

实验方案

Product Specific Protocols
WB protocol for AP4M1 antibody 11653-1-APDownload protocol
IHC protocol for AP4M1 antibody 11653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma

Authors - Yuanhao Peng
View Article