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AP4S1 Polyclonal antibody

AP4S1 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27202-1-AP

Print datasheet

Synonyms

AP 4 complex subunit sigma 1, AP47B, AP4S1, CLA20, CLAPS4, Sigma 1 subunit of AP 4, Sigma 4 adaptin, Sigma4 adaptin

验证数据展示

  • IF Staining of mouse brain using 27202-1-AP

    IF Staining of mouse brain using 27202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 27202-1-AP

    IHC staining of mouse brain using 27202-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27202-1-AP

    IHC staining of mouse brain using 27202-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27202-1-AP

    IF Staining of mouse brain using 27202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27202-1-AP targets AP4S1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen AP4S1 fusion protein Ag25661 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 4, sigma 1 subunit
Synonyms AP 4 complex subunit sigma 1, AP47B, AP4S1, CLA20, CLAPS4, Sigma 1 subunit of AP 4, Sigma 4 adaptin, Sigma4 adaptin
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC001259
Gene Symbol AP4S1
Gene ID (NCBI) 11154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y587
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AP4S1 antibody 27202-1-APDownload protocol
IF protocol for AP4S1 antibody 27202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols