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AP5Z1/SPG48 Monoclonal antibody

AP5Z1/SPG48 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1D7

Cat no : 66533-1-Ig

Print datasheet

Synonyms

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

验证数据展示

  • WB analysis using 66533-1-Ig

    WB analysis using 66533-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66533-1-Ig

    IF Staining of HEK-293 using 66533-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AP5Z1/SPG48 antibody (66533-1-Ig, Clone: 1G1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human liver cancer using 66533-1-Ig

    IHC staining of human liver cancer using 66533-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, Caco-2 cells, COLO 320 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66533-1-Ig targets AP5Z1/SPG48 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen AP5Z1/SPG48 fusion protein Ag26961 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA0415
Synonyms AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5
Calculated Molecular Weight 807 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC037399
Gene Symbol AP5Z1/SPG48
Gene ID (NCBI) 9907
RRIDAB_2881896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.

实验方案

Product Specific Protocols
WB protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
IHC protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
IF protocol for AP5Z1/SPG48 antibody 66533-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols