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AP5Z1/SPG48 Monoclonal antibody, PBS Only

AP5Z1/SPG48 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1D7

Cat no : 66533-1-PBS

Print datasheet

Synonyms

AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5

验证数据展示

  • WB analysis using 66533-1-Ig (same clone as 66533-1-PBS)

    WB analysis using 66533-1-Ig (same clone as 66533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66533-1-Ig (Zeta5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66533-1-Ig (same clone as 66533-1-PBS)

    IF Staining of HEK-293 using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AP5Z1/SPG48 antibody (66533-1-Ig, Clone: 1G1D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

    IHC staining of human liver cancer using 66533-1-Ig (same clone as 66533-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66533-1-Ig (AP5Z1/SPG48 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66533-1-PBS in a different storage buffer formulation.

产品信息

66533-1-PBS targets AP5Z1/SPG48 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen AP5Z1/SPG48 fusion protein Ag26961 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name KIAA0415
Synonyms AP-5 complex subunit zeta-1, AP5Z1, AP5Z1/SPG48, KIAA0415, SPG48, Zeta5
Calculated Molecular Weight 807 aa, 88 kDa
Observed Molecular Weight 88 kDa, 160 kDa
GenBank Accession NumberBC037399
Gene Symbol AP5Z1/SPG48
Gene ID (NCBI) 9907
RRIDAB_2881896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43299
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.