APAF1 Polyclonal antibody

APAF1 Polyclonal Antibody for WB, ELISA
Cat No. 29022-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (2)

应用

WB, ELISA

APAF 1, APAF1, CED4, DKFZp781B1145, KIAA0413

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

29022-1-AP targets APAF1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human, rat
免疫原 APAF1 fusion protein Ag30606 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptotic peptidase activating factor 1
别名 APAF 1, APAF1, CED4, DKFZp781B1145, KIAA0413
计算分子量 1248 aa, 142 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC136532
基因名称 APAF1
Gene ID (NCBI) 317
RRIDAB_2923588
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14727
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

实验方案

Product Specific Protocols
WB protocol for APAF1 antibody 29022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Nutrients

Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats

Authors - Yujie Zhong
humanWB

J Adv Res

Sodium citrate targeting Ca2+/CAMKK2 pathway exhibits anti-tumor activity through inducing apoptosis and ferroptosis in ovarian cancer

Authors - Yulun Wu
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