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验证数据展示

  • WB analysis using 55089-1-AP

    WB analysis using 55089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55089-1-AP (APBA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 55089-1-AP

    IHC staining of mouse brain using 55089-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55089-1-AP (APBA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBA1 antibody included
  • IHC staining of mouse brain using 55089-1-AP

    IHC staining of mouse brain using 55089-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55089-1-AP (APBA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APBA1 antibody included

APBA1 Polyclonal antibody

APBA1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

X11, Neuron specific X11 protein, MINT1, Mint 1, D9S411E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells, SH-SY5Y cells, SK-N-SH cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy APBA1 Ready-To-Use IHC Kit. APBA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55089-1-AP targets APBA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 amyloid beta (A4) precursor protein-binding, family A, member 1
别名 X11, Neuron specific X11 protein, MINT1, Mint 1, D9S411E
计算分子量 93 kDa
观测分子量138-140 kDa
GenBank蛋白编号NM_001163
基因名称 APBA1
Gene ID (NCBI) 320
RRIDAB_3670237
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02410
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APBA1, also named MINT1 and X11A, is a brain-specific multi-modular protein that interacts with the Alzheimer's disease amyloid precursor protein (APP)(PMID: 22136355). APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. APBA1 is expressed in the brain and was found to interact with Munc18.

实验方案

Product Specific Protocols
WB protocol for APBA1 antibody 55089-1-APDownload protocol
IHC protocol for APBA1 antibody 55089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols