• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

APBB1 Polyclonal antibody, PBS Only

APBB1 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27874-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 27874-1-AP (same clone as 27874-1-PBS)

    WB analysis using 27874-1-AP (same clone as 27874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27874-1-AP (APBB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 27874-1-AP (same clone as 27874-1-PBS)

    IP experiment of mouse brain using 27874-1-AP (same clone as 27874-1-PBS)

    IP result of anti-APBB1 (IP:27874-1-AP, 4ug; Detection:27874-1-AP 1:3000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy APBB1 Ready-To-Use IHC Kit. APBB1 primary antibody included.

产品信息

27874-1-PBS targets APBB1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen APBB1 fusion protein Ag27434 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Synonyms
Calculated Molecular Weight 708 aa, 77 kDa
Observed Molecular Weight 65 kDa, 97 kDa
GenBank Accession NumberBC010854
Gene Symbol APBB1
Gene ID (NCBI) 322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Adaptor protein FE65 (APBB1) specifically binds to the intracellular tail of the type I transmembrane protein, β-amyloid precursor protein (APP). FE65 is a multimodular adaptor protein, consisting of three major protein-protein interaction domains, a WW domain and two phosphotyrosine interaction domains (PID). The interaction between FE65 and APP mainly takes place between the C-terminal PID (PID2) and the APP intracellular domain (AICD) (PMID: 15647266). There is novel evidence that the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis. The cleavage region locates immediately after an acidic residue cluster but before the three major protein-protein binding domains (PMID: 15647266). FE65 is predominantly expressed in central nervous system neurons, and its expression is regulated during development and aging, with high levels corresponding to the timing of neural tissue formation and high neuronal activity (PMID: 7867517, PMID: 10561691, PMID: 14689444). p65FE65 (65kDa) is endoproteolytically cleaved from p97FE65 (97 kDa), the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis (PMID: 15647266).