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APBB1 Monoclonal antibody, PBS Only

APBB1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9G10

Cat no : 67077-1-PBS

Print datasheet

Synonyms

APBB1, FE65, Protein Fe65, RIR

验证数据展示

  • WB analysis using 67077-1-Ig (same clone as 67077-1-PBS)

    WB analysis using 67077-1-Ig (same clone as 67077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67077-1-Ig (APBB1 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APBB1 antibody included
  • IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APBB1 antibody included
  • IF Staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    IF Staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using APBB1 antibody (67077-1-Ig, Clone: 1B9G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy APBB1 Ready-To-Use IHC Kit. APBB1 primary antibody included.

产品信息

67077-1-PBS targets APBB1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen APBB1 fusion protein Ag27436 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Synonyms APBB1, FE65, Protein Fe65, RIR
Calculated Molecular Weight 708 aa, 77 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC010854
Gene Symbol APBB1
Gene ID (NCBI) 322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).