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APBB1 Monoclonal antibody, PBS Only

APBB1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B9G10

Cat no : 67077-1-PBS

Print datasheet

Synonyms

APBB1, FE65, Protein Fe65, RIR

验证数据展示

  • WB analysis using 67077-1-Ig (same clone as 67077-1-PBS)

    WB analysis using 67077-1-Ig (same clone as 67077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67077-1-Ig (APBB1 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APBB1 antibody included
  • IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    IHC staining of mouse cerebellum using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67077-1-Ig (APBB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APBB1 antibody included
  • IF Staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    IF Staining of mouse brain using 67077-1-Ig (same clone as 67077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using APBB1 antibody (67077-1-Ig, Clone: 1B9G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67077-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy APBB1 Ready-To-Use IHC Kit. APBB1 primary antibody included.

产品信息

67077-1-PBS targets APBB1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen APBB1 fusion protein Ag27436 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Synonyms APBB1, FE65, Protein Fe65, RIR
Calculated Molecular Weight 708 aa, 77 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC010854
Gene Symbol APBB1
Gene ID (NCBI) 322
RRIDAB_2882385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00213
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239).