Serum amyloid P component Monoclonal antibody
Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1A1D10
验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, human plasma |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue, human liver tissue, mouse spleen tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
66084-1-Ig targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | Serum amyloid P component fusion protein Ag14957 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | amyloid P component, serum |
| Synonyms | APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein |
| Calculated Molecular Weight | 223 aa, 25 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC007058 |
| Gene Symbol | Serum amyloid P component |
| Gene ID (NCBI) | 325 |
| RRID | AB_11182271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02743 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Serum amyloid P component antibody 66084-1-Ig | Download protocol |
| IHC protocol for Serum amyloid P component antibody 66084-1-Ig | Download protocol |
| IF protocol for Serum amyloid P component antibody 66084-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
