验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
25689-1-AP targets API5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | API5 fusion protein Ag22524 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | apoptosis inhibitor 5 |
| Synonyms | AAC 11, AAC11, Antiapoptosis clone 11 protein, API 5, API5, apoptosis inhibitor 5, FIF, Protein XAGL |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC017709 |
| Gene Symbol | API5 |
| Gene ID (NCBI) | 8539 |
| RRID | AB_2880196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human gene API5 encodes the protein Apoptosis inhibitor 5. This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of chemotherapeutic drugs. Diseases associated with API5 include colon adenocarcinoma, and cervical cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for API5 antibody 25689-1-AP | Download protocol |
| IHC protocol for API5 antibody 25689-1-AP | Download protocol |
| IF protocol for API5 antibody 25689-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gene Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica. | ||
Oncol Lett Paeonol-mediated apoptosis of hepatocellular carcinoma cells by NF-κB pathway. |
