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Apolipoprotein AI Recombinant antibody, PBS Only

Apolipoprotein AI Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240269F1

Cat no : 83504-1-PBS

Print datasheet

Synonyms

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

验证数据展示

  • WB analysis of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    WB analysis of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83504-1-RR (same clone as 83504-1-PBS)

    IHC staining of human stomach cancer using 83504-1-RR (same clone as 83504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA1 antibody included
  • FC experiment of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    FC experiment of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Apolipoprotein AI Recombinant antibody (83504-1-RR, Clone:240269F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83504-1-RR

    Affinity and Kinetic Characterization of 83504-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83504-1-RR against Human Apolipoprotein AI were performed. The affinity constant is 4.05 nM.

Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.

产品信息

83504-1-PBS targets Apolipoprotein AI in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name apolipoprotein A-I
Synonyms APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02647
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis.