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Apolipoprotein AI Recombinant antibody, PBS Only

Apolipoprotein AI Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240269F1

Cat no : 83504-1-PBS

Print datasheet

Synonyms

APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I

验证数据展示

  • WB analysis of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    WB analysis of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83504-1-RR (same clone as 83504-1-PBS)

    IHC staining of human stomach cancer using 83504-1-RR (same clone as 83504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOA1 antibody included
  • FC experiment of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    FC experiment of HepG2 using 83504-1-RR (same clone as 83504-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Apolipoprotein AI Recombinant antibody (83504-1-RR, Clone:240269F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83504-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83504-1-RR

    Affinity and Kinetic Characterization of 83504-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83504-1-RR against Human Apolipoprotein AI were performed. The affinity constant is 4.05 nM.

Planning an IHC experiment? We recommend our IHCeasy APOA1 Ready-To-Use IHC Kit. APOA1 primary antibody included.

产品信息

The immunogen of 83504-1-PBS is Apolipoprotein AI Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name apolipoprotein A-I
Synonyms APOA1, apolipoprotein A I, Apo-AI, ApoAI, ApoA I
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP02647
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis.