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APOB Polyclonal antibody, PBS Only

APOB Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20578-1-PBS

Print datasheet

Synonyms

Apolipoprotein B, Apolipoprotein B-100, ApoB48, ApoB100, Apo B-100

验证数据展示

  • WB analysis using 20578-1-AP (same clone as 20578-1-PBS)

    WB analysis using 20578-1-AP (same clone as 20578-1-PBS)

    mouse liver and human plasma were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

  • WB analysis using 20578-1-AP (same clone as 20578-1-PBS)

    WB analysis using 20578-1-AP (same clone as 20578-1-PBS)

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 20578-1-AP (same clone as 20578-1-PBS)

    IHC staining of human liver using 20578-1-AP (same clone as 20578-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 20578-1-AP (same clone as 20578-1-PBS)

    IHC staining of human liver using 20578-1-AP (same clone as 20578-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 20578-1-AP (same clone as 20578-1-PBS)

    IHC staining of human hepatocirrhosis using 20578-1-AP (same clone as 20578-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 20578-1-AP (same clone as 20578-1-PBS)

    IHC staining of human hepatocirrhosis using 20578-1-AP (same clone as 20578-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

产品信息

20578-1-PBS targets APOB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein B (including Ag(x) antigen)
Synonyms Apolipoprotein B, Apolipoprotein B-100, ApoB48, ApoB100, Apo B-100
Calculated Molecular Weight 516 kDa
Observed Molecular Weight150-250 kDa, 400-520 kDa
GenBank Accession NumberNM_000384
Gene Symbol APOB
Gene ID (NCBI) 338
RRIDAB_10732938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04114
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).