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APOBEC2 Polyclonal antibody, PBS Only

APOBEC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20121-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 20121-1-AP (same clone as 20121-1-PBS)

    WB analysis using 20121-1-AP (same clone as 20121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20121-1-AP (APOBEC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

    IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

    IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse skeletal muscle using 20121-1-AP (same clone as 20121-1-PBS)

    IP experiment of mouse skeletal muscle using 20121-1-AP (same clone as 20121-1-PBS)

    IP result of anti-APOBEC2 (IP:20121-1-AP, 4ug; Detection:20121-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

产品信息

20121-1-PBS targets APOBEC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen APOBEC2 fusion protein Ag13699 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
Synonyms
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC047767
Gene Symbol APOBEC2
Gene ID (NCBI) 10930
RRIDAB_10697687
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y235
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APOBEC2, also named as ARCD1 and ARP1, is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide (APOBEC) cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. APOBEC family members have diverse roles by their ability to edit DNA and/or RNA. Apobec2 is one of the oldest members of the APOBEC family, along with the lymphoid-specific activation-induced deaminase. Unlike other APOBEC family members, the enzymatic activity and substrate of Apobec2 have not been fully demonstrated, and its biologic functions remain unknown. It can be detected as 25-30 kDa.