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验证数据展示

  • WB analysis using 25084-1-AP

    WB analysis using 25084-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25084-1-AP (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 25084-1-AP

    IF Staining of THP-1 using 25084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (25084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

APOBEC3A Polyclonal antibody

APOBEC3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Phorbolin-1, PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHaCaT cells, HeLa cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25084-1-AP targets APOBEC3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 APOBEC3A fusion protein Ag18845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
别名 Phorbolin-1, PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3
计算分子量 199 aa, 23 kDa
观测分子量23 kDa
GenBank蛋白编号BC126416
基因名称 APOBEC3A
Gene ID (NCBI) 200315
RRIDAB_3085769
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.

实验方案

Product Specific Protocols
WB protocol for APOBEC3A antibody 25084-1-APDownload protocol
IF protocol for APOBEC3A antibody 25084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols