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APOBEC3A Polyclonal antibody

APOBEC3A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 25084-1-AP

Print datasheet

Synonyms

Phorbolin-1, PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3

验证数据展示

  • WB analysis using 25084-1-AP

    WB analysis using 25084-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25084-1-AP (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 25084-1-AP

    IF Staining of THP-1 using 25084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (25084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHaCaT cells, HeLa cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25084-1-AP targets APOBEC3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen APOBEC3A fusion protein Ag18845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Synonyms Phorbolin-1, PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3
Calculated Molecular Weight 199 aa, 23 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC126416
Gene Symbol APOBEC3A
Gene ID (NCBI) 200315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.

实验方案

Product Specific Protocols
WB protocol for APOBEC3A antibody 25084-1-APDownload protocol
IF protocol for APOBEC3A antibody 25084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols