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验证数据展示

  • WB analysis using 84239-4-RR

    WB analysis using 84239-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84239-4-RR (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84239-4-RR

    FC experiment of A431 using 84239-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Apobec3a Recombinant Antibody (84239-4-RR, Clone:241529G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84239-4-RR

    Affinity and Kinetic Characterization of 84239-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-4-RR against Human APOBEC3A were performed. The affinity constant is 0.328 nM.

APOBEC3A Recombinant antibody

APOBEC3A Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84239-4-RR

产品说明书

CloneNo. 241529G4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat colon tissue, HeLa cells, HaCaT cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84239-4-RR targets APOBEC3A in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 APOBEC3A fusion protein Ag18845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
别名 PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A
计算分子量 199 aa, 23 kDa
观测分子量28 kDa
GenBank蛋白编号BC126416
基因名称 APOBEC3A
Gene ID (NCBI) 200315
RRIDAB_3671792
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31941
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.

实验方案

Product Specific Protocols
WB protocol for APOBEC3A antibody 84239-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols