验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14559-1-AP targets APOBEC3B in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | APOBEC3B fusion protein Ag6115 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B |
| 别名 | A3B, APOBEC1L, APOBEC3B, ARCD3, ARP4, bK150C2.2, Phorbolin 1 related protein, Phorbolin 2/3, PHRBNL |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC053859 |
| 基因名称 | APOBEC3B |
| Gene ID (NCBI) | 9582 |
| RRID | AB_2878066 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UH17 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The DNA cytosine deaminase APOBEC3B was identified recently as a source of DNA damage and mutagenesis in breast, head/neck, cervix, bladder, lung, ovary, and to lesser extents additional cancer types. This enzyme is also an effector protein in the innate immune response to virus infection. APOBEC3B has 3 isoforms produced by alternative splicing with the MW of 46, 29, 43 kDa. It can also exist as a dimer and can interact with APOBEC3G.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APOBEC3B antibody 14559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Genet Multiregion whole-genome sequencing depicts intratumour heterogeneity and punctuated evolution in ovarian clear cell carcinoma. | ||
Front Oncol Correlation Between APOBEC3B Expression and Clinical Characterization in Lower-Grade Gliomas. | ||
Mol Carcinog HPV-16/18 E6-induced APOBEC3B expression associates with proliferation of cervical cancer cells and hypomethylation of Cyclin D1. | ||
Zhongguo Fei Ai Za Zhi [SWI/SNF Complex Gene Mutations Promote the Liver Metastasis of Non-small Cell Lung Cancer Cells in NSI Mice] |
