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APOF Polyclonal antibody

APOF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16608-1-AP

Print datasheet

Synonyms

Apo F, APOF, apolipoprotein F, LTIP

验证数据展示

  • WB analysis of human plasma using 16608-1-AP

    WB analysis of human plasma using 16608-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 16608-1-AP (APOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16608-1-AP

    IHC staining of human liver using 16608-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16608-1-AP (APOF antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16608-1-AP

    IHC staining of human liver using 16608-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16608-1-AP (APOF antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman plasma tissue, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16608-1-AP targets APOF in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen APOF fusion protein Ag9824 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein F
Synonyms Apo F, APOF, apolipoprotein F, LTIP
Calculated Molecular Weight 326 aa, 35 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC026257
Gene Symbol APOF
Gene ID (NCBI) 319
RRIDAB_2058253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APOF antibody 16608-1-APDownload protocol
IHC protocol for APOF antibody 16608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.

Authors - Zijian Da
View Article