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APOH Recombinant antibody, PBS Only (Detector)

APOH Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242454E4

Cat no : 84943-2-PBS

Print datasheet

Synonyms

Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

验证数据展示

  • WB analysis of human plasma using 84943-2-RR (same clone as 84943-2-PBS)

    WB analysis of human plasma using 84943-2-RR (same clone as 84943-2-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84943-2-RR (APOH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84943-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01667-1

    Sandwich ELISA standard curve of MP01667-1

    Sandwich ELISA standard curve of MP01667-1, Human APOH Recombinant Matched Antibody Pair - PBS only. 84943-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3084. 84943-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • Sample test of MP01667-1

    Sample test of MP01667-1

    Plasma of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 51.16 µg/mL with a range of 38.03 - 72.18 µg/mL.

  • Sample test of MP01667-1

    Sample test of MP01667-1

    The mean APOH concentration was determined to be 21.74 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • Sample test of MP01667-1

    Sample test of MP01667-1

    Serum of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 69.62 µg/mL with a range of 55.50 - 88.74 µg/mL.

  • Cytometric bead array standard curve of MP01667-1

    Cytometric bead array standard curve of MP01667-1

    Cytometric bead array standard curve of MP01667-1, APOH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84943-1-PBS. Detection antibody: 84943-2-PBS. Standard: Eg3084. Range: 0.625-80 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.

产品信息

84943-2-PBS targets APOH as part of a matched antibody pair:

MP01667-1: 84943-1-PBS capture and 84943-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name apolipoprotein H (beta-2-glycoprotein I)
Synonyms Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein
Calculated Molecular Weight38 kDa
Observed Molecular Weight 43-55 kDa
GenBank Accession NumberNM_000042.2
Gene Symbol APOH
Gene ID (NCBI) 350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.