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APOL1 Polyclonal antibody

APOL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11486-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells, HepG2 cells, A2780 cells, human plasma, SW 1990 cells, A549 cells, SKOV-3 cells, HeLa cells, MCF-7 cells
Positive IP detected inHepG2 cells, human plasma tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human pancreas tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11486-2-AP targets APOL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human
文献引用反应性human, trypanosoma brucei
免疫原 APOL1 fusion protein Ag2016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein L, 1
别名 Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L
计算分子量 44 kDa
观测分子量 39 kDa
GenBank蛋白编号BC017331
基因名称 APOL1
Gene ID (NCBI) 8542
RRIDAB_2058396
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14791
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.

实验方案

Product Specific Protocols
WB protocol for APOL1 antibody 11486-2-APDownload protocol
IHC protocol for APOL1 antibody 11486-2-APDownload protocol
IF protocol for APOL1 antibody 11486-2-APDownload protocol
IP protocol for APOL1 antibody 11486-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Med

Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.

Authors - Molina-Portela Maria Pilar MP
humanWB

Proc Natl Acad Sci U S A

Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.

Authors - Thomson Russell R
WB

Proc Natl Acad Sci U S A

Evolution of the primate trypanolytic factor APOL1.

Authors - Russell Thomson
humanWB

J Am Soc Nephrol

Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.

Authors - Nidhi Gupta
  • KD Validated
humanWB,IF

J Am Soc Nephrol

Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.

Authors - Suzie J Scales
  • KO Validated
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
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