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验证数据展示

  • IF Staining of HepG2 using 83770-5-RR

    IF Staining of HepG2 using 83770-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1 antibody (83770-5-RR, Clone: 240814D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 83770-5-RR

    WB analysis using 83770-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83770-5-RR (APOL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83770-5-RR

    Affinity and Kinetic Characterization of 83770-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83770-5-RR against Human APOL1 were performed. The affinity constant is below 1 pM.

APOL1 Recombinant antibody

APOL1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83770-5-RR

产品说明书

CloneNo. 240814D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A375 cells, HepG2 cells, MCF-7 cells, human plasma
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83770-5-RR targets APOL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 APOL1 fusion protein ag2016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein L, 1
别名 Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11
计算分子量 44 kDa
观测分子量 39 kDa
GenBank蛋白编号BC017331
基因名称 APOL1
Gene ID (NCBI) 8542
RRIDAB_3671364
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO14791
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue.

实验方案

Product Specific Protocols
WB protocol for APOL1 antibody 83770-5-RRDownload protocol
IF protocol for APOL1 antibody 83770-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols