验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A375 cells, HepG2 cells, MCF-7 cells, human plasma |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83770-5-RR targets APOL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | APOL1 fusion protein ag2016 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | apolipoprotein L, 1 |
| Synonyms | Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC017331 |
| Gene Symbol | APOL1 |
| Gene ID (NCBI) | 8542 |
| RRID | AB_3671364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | O14791 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APOL1 antibody 83770-5-RR | Download protocol |
| IF protocol for APOL1 antibody 83770-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |