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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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APOL1 Recombinant antibody

APOL1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240814D11

Cat no : 83770-5-RR

Print datasheet

Synonyms

Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11

验证数据展示

  • IF Staining of HepG2 using 83770-5-RR

    IF Staining of HepG2 using 83770-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1 antibody (83770-5-RR, Clone: 240814D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 83770-5-RR

    WB analysis using 83770-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83770-5-RR (APOL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83770-5-RR

    Affinity and Kinetic Characterization of 83770-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83770-5-RR against Human APOL1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA431 cells, A375 cells, HepG2 cells, MCF-7 cells, human plasma
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83770-5-RR targets APOL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen APOL1 fusion protein ag2016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name apolipoprotein L, 1
Synonyms Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC017331
Gene Symbol APOL1
Gene ID (NCBI) 8542
RRIDAB_3671364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14791
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue.

实验方案

Product Specific Protocols
WB protocol for APOL1 antibody 83770-5-RRDownload protocol
IF protocol for APOL1 antibody 83770-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols