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  • Featured Product
  • KD/KO Validated

APOL1-Specific Monoclonal antibody, PBS Only

APOL1-Specific Monoclonal Antibody for WB,IP,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

1G12D11

Cat No : 66124-1-PBS

Print datasheet

Synonyms

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

验证数据展示

  • IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

    IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOL1 antibody included
  • WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    1.5 ug human plasma was subjected to SDS PAGE followed by western blot with 66124-1-Ig (APOL1 Antibody) at dilution of 1:400, 000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    IP result of anti-APOL1-Specific (IP:66124-1-Ig, 5ug; Detection:66124-1-Ig 1:10000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

    IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

    FC experiment of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

    1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (66124-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOL1 antibody included
  • IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOL1 antibody included
Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.

产品信息

66124-1-PBS targets APOL1-Specific in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,IHC,IF,FC,ELISA Application Description
Tested Reactivity human
Immunogen APOL1-Specific fusion protein Ag2016 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name apolipoprotein L, 1
Synonyms APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberBC017331
Gene Symbol APOL1
Gene ID (NCBI) 8542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14791
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.