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验证数据展示

  • IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

    IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOL1 antibody included
  • WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    1.5 ug human plasma was subjected to SDS PAGE followed by western blot with 66124-1-Ig (APOL1 Antibody) at dilution of 1:400, 000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

    IP result of anti-APOL1-Specific (IP:66124-1-Ig, 5ug; Detection:66124-1-Ig 1:10000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOL1 antibody included
  • IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOL1 antibody included
  • IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

    IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1-Specific antibody (66124-1-Ig, Clone: 1G12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

APOL1-Specific Monoclonal antibody, PBS Only

APOL1-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66124-1-PBS

产品说明书

CloneNo. 1G12D11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy APOL1 Ready-To-Use IHC Kit. APOL1 primary antibody included.

产品信息

66124-1-PBS targets APOL1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 APOL1-Specific fusion protein Ag2016 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein L, 1
别名 APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1
计算分子量 44 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC017331
基因名称 APOL1
Gene ID (NCBI) 8542
RRIDAB_2881523
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14791
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.