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- KD/KO Validated
APOL2 Polyclonal antibody
APOL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, A431 cells, HeLa cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
产品信息
25925-1-AP targets APOL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | APOL2 fusion protein Ag7622 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | apolipoprotein L, 2 |
Synonyms | Apolipoprotein L-II, ApoL-II, APOL3, APOL II |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC004395 |
Gene Symbol | APOL2 |
Gene ID (NCBI) | 23780 |
RRID | AB_2880297 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9BQE5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for APOL2 antibody 25925-1-AP | Download protocol |
IHC protocol for APOL2 antibody 25925-1-AP | Download protocol |
IF protocol for APOL2 antibody 25925-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Chem Commun (Camb) Competitive affinity-based proteome profiling and imaging to reveal potential cellular targets of betulinic acid.
| ||
Aging (Albany NY) TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs) |