• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

APOM Recombinant antibody

APOM Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3O19

Cat no : 82871-1-RR

Print datasheet

Synonyms

HSPC336, G3A, Apo-M, Apolipoprotein M, Apo M

验证数据展示

  • WB analysis using 82871-1-RR

    WB analysis using 82871-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82871-1-RR (APOM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82871-1-RR

    IF Staining of HepG2 using 82871-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (82871-1-RR, Clone: 3O19 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HepG2 using 82871-1-RR

    FC experiment of HepG2 using 82871-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOM Recombinant antibody (82871-1-RR, Clone:3O19) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82871-1-RR

    Affinity and Kinetic Characterization of 82871-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82871-1-RR against Human APOM were performed. The affinity constant is 0.694 nM.

经过测试的应用

Positive WB detected inHepG2 cells, human plasma
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82871-1-RR targets APOM in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen APOM fusion protein Ag3048 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name apolipoprotein M
Synonyms HSPC336, G3A, Apo-M, Apolipoprotein M, Apo M
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-25 kDa
GenBank Accession NumberBC020683
Gene Symbol APOM
Gene ID (NCBI) 55937
RRIDAB_3670591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95445
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

实验方案

Product Specific Protocols
WB protocol for APOM antibody 82871-1-RRDownload protocol
IF protocol for APOM antibody 82871-1-RRDownload protocol
FC protocol for APOM antibody 82871-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols