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验证数据展示

  • WB analysis using 28514-1-AP

    WB analysis using 28514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28514-1-AP (APOOL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28514-1-AP

    IHC staining of human kidney using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human kidney using 28514-1-AP

    IHC staining of human kidney using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human placenta using 28514-1-AP

    IHC staining of human placenta using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human placenta using 28514-1-AP

    IHC staining of human placenta using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IF Staining of HeLa using 28514-1-AP

    IF Staining of HeLa using 28514-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using APOOL antibody (28514-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

APOOL Polyclonal antibody

APOOL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28514-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

apolipoprotein O like, APOOL, CXorf33, FAM121A, MIC27, Protein FAM121A, UNQ8193

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy APOOL Ready-To-Use IHC Kit. APOOL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28514-1-AP targets APOOL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 APOOL fusion protein Ag29392 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein O-like
别名 apolipoprotein O like, APOOL, CXorf33, FAM121A, MIC27, Protein FAM121A, UNQ8193
计算分子量 268 aa, 29 kDa
观测分子量30 kDa
GenBank蛋白编号BC107096
基因名称 APOOL
Gene ID (NCBI) 139322
RRIDAB_3086061
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOOL, the component of the MICOS complex, is a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979). Its molecular weight is about 30 kDa.

实验方案

Product Specific Protocols
WB protocol for APOOL antibody 28514-1-APDownload protocol
IHC protocol for APOOL antibody 28514-1-APDownload protocol
IF protocol for APOOL antibody 28514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols