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APOOL Polyclonal antibody

APOOL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28514-1-AP

Print datasheet

Synonyms

apolipoprotein O like, APOOL, CXorf33, FAM121A, MIC27, Protein FAM121A, UNQ8193

验证数据展示

  • WB analysis using 28514-1-AP

    WB analysis using 28514-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28514-1-AP (APOOL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28514-1-AP

    IHC staining of human kidney using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human kidney using 28514-1-AP

    IHC staining of human kidney using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human placenta using 28514-1-AP

    IHC staining of human placenta using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IHC staining of human placenta using 28514-1-AP

    IHC staining of human placenta using 28514-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with APOOL antibody included
  • IF Staining of HeLa using 28514-1-AP

    IF Staining of HeLa using 28514-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using APOOL antibody (28514-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy APOOL Ready-To-Use IHC Kit. APOOL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28514-1-AP targets APOOL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen APOOL fusion protein Ag29392 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein O-like
Synonyms apolipoprotein O like, APOOL, CXorf33, FAM121A, MIC27, Protein FAM121A, UNQ8193
Calculated Molecular Weight 268 aa, 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC107096
Gene Symbol APOOL
Gene ID (NCBI) 139322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

APOOL, the component of the MICOS complex, is a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979). Its molecular weight is about 30 kDa.

实验方案

Product Specific Protocols
WB protocol for APOOL antibody 28514-1-APDownload protocol
IHC protocol for APOOL antibody 28514-1-APDownload protocol
IF protocol for APOOL antibody 28514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols