验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12409-1-AP targets APPBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Immunogen | APPBP2 fusion protein Ag3092 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | amyloid beta precursor protein (cytoplasmic tail) binding protein 2 |
| Synonyms | APP BP2, APPBP2, HS.84084, KIAA0228, PAT1 |
| Calculated Molecular Weight | 585 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC018121 |
| Gene Symbol | APPBP2 |
| Gene ID (NCBI) | 10513 |
| RRID | AB_2918029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92624 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APPBP2 antibody 12409-1-AP | Download protocol |
| IHC protocol for APPBP2 antibody 12409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
