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APPBP2 Polyclonal antibody

APPBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12409-1-AP

Print datasheet

Synonyms

APP BP2, APPBP2, HS.84084, KIAA0228, PAT1

验证数据展示

  • WB analysis using 12409-1-AP

    WB analysis using 12409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12409-1-AP

    IHC staining of human breast cancer using 12409-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

经过测试的应用

Positive WB detected inA2780 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12409-1-AP targets APPBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen APPBP2 fusion protein Ag3092 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amyloid beta precursor protein (cytoplasmic tail) binding protein 2
Synonyms APP BP2, APPBP2, HS.84084, KIAA0228, PAT1
Calculated Molecular Weight 585 aa, 67 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC018121
Gene Symbol APPBP2
Gene ID (NCBI) 10513
RRIDAB_2918029
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92624
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).

实验方案

Product Specific Protocols
WB protocol for APPBP2 antibody 12409-1-APDownload protocol
IHC protocol for APPBP2 antibody 12409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Diabetes

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Authors - Yi Zhong
View Article