APPL2 Polyclonal antibody
APPL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells |
| Positive IHC detected in | mouse brain tissue, human malignant melanoma tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy APPL2 Ready-To-Use IHC Kit. APPL2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
14294-1-AP targets APPL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | APPL2 fusion protein Ag5626 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 |
| Synonyms | Dip13-beta, DIP13B, Dip13 beta, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2 |
| Calculated Molecular Weight | 74 kDa |
| Observed Molecular Weight | 74 kDa |
| GenBank Accession Number | BC033731 |
| Gene Symbol | APPL2 |
| Gene ID (NCBI) | 55198 |
| RRID | AB_2878041 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEU8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APPL2 antibody 14294-1-AP | Download protocol |
| IHC protocol for APPL2 antibody 14294-1-AP | Download protocol |
| IF protocol for APPL2 antibody 14294-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle Physical exercise attenuates age-related muscle atrophy and exhibits anti-ageing effects via the adiponectin receptor 1 signalling | ||
J Immunol Long Noncoding RNA HAFML Promotes Migration and Invasion of Rheumatoid Fibroblast-like Synoviocytes | ||
J Cell Sci GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region | ||
bioRxiv GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region |
