验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells, K-562 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
10680-1-AP targets APRIL,TNFSF13 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | APRIL,TNFSF13 fusion protein Ag1056 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor (ligand) superfamily, member 13 |
| 别名 | TNFSF13, TNF- and APOL-related leukocyte expressed ligand 2, TALL-2, TALL2, CD256 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC008042 |
| 基因名称 | TNFSF13 |
| Gene ID (NCBI) | 8741 |
| RRID | AB_2206515 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75888 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APRIL,TNFSF13 antibody 10680-1-AP | Download protocol |
| IHC protocol for APRIL,TNFSF13 antibody 10680-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol TNFSF13 Is a Novel Onco-Inflammatory Marker and Correlates With Immune Infiltration in Gliomas. | ||
Clin Biochem APRIL knockdown suppresses migration and invasion of human colon carcinoma cells.
| ||
Pathol Oncol Res Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway.
|
