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  • Featured Product
  • KD/KO Validated

APRT Polyclonal antibody

APRT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 21405-1-AP

Print datasheet

Synonyms

AMP, APRT

验证数据展示

  • WB analysis of A549 using 21405-1-AP

    WB analysis of A549 using 21405-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21405-1-AP (APRT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 21405-1-AP

    IF Staining of A549 using 21405-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 21405-1-AP (APRT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, A431 cells
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21405-1-AP targets APRT in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen APRT fusion protein Ag16119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenine phosphoribosyltransferase
Synonyms AMP, APRT
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC106894
Gene Symbol APRT
Gene ID (NCBI) 353
RRIDAB_10888630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APRT antibody 21405-1-APDownload protocol
IF protocol for APRT antibody 21405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.

Authors - Xiaoting Chen
View Article
human,mouseWB

Nat Commun

Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas

Authors - Jianxing Yin
  • KD Validated
View Article