AQP2 Polyclonal antibody
AQP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | 37°C incubated mouse kidney tissue |
Positive IHC detected in | mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
29386-1-AP targets AQP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | Human, mouse |
Cited Reactivity | mouse |
Immunogen | AQP2 fusion protein Ag29931 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aquaporin 2 (collecting duct) |
Synonyms | ADH water channel, AQP CD, AQP2, AQP-2, Aquaporin 2, aquaporin 2 (collecting duct), Aquaporin CD, WCH CD |
Calculated Molecular Weight | 29 kDa |
Observed Molecular Weight | 28-29, 35-38 kDa |
GenBank Accession Number | NM_000486 |
Gene Symbol | AQP2 |
Gene ID (NCBI) | 359 |
RRID | AB_2918288 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P41181 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AQP2 antibody 29386-1-AP | Download protocol |
IHC protocol for AQP2 antibody 29386-1-AP | Download protocol |
IF protocol for AQP2 antibody 29386-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |