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验证数据展示

  • IHC staining of mouse kidney using 29386-1-AP

    IHC staining of mouse kidney using 29386-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AQP2 antibody included
  • IF Staining of mouse kidney using 29386-1-AP

    IF Staining of mouse kidney using 29386-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), AQP1 antibody (66805-1-Ig, Clone: 2E5D6, green).

  • WB analysis of mouse kidney using 29386-1-AP

    WB analysis of mouse kidney using 29386-1-AP

    37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29386-1-AP (AQP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AQP2 Polyclonal antibody

AQP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29386-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF-P, ELISA and More (1)

别名

ADH water channel, AQP CD, AQP2, AQP-2, Aquaporin 2, aquaporin 2 (collecting duct), Aquaporin CD, WCH CD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in37°C incubated mouse kidney tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy AQP2 Ready-To-Use IHC Kit. AQP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29386-1-AP targets AQP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 Human, mouse
文献引用反应性mouse
免疫原 AQP2 fusion protein Ag29931 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aquaporin 2 (collecting duct)
别名 ADH water channel, AQP CD, AQP2, AQP-2, Aquaporin 2, aquaporin 2 (collecting duct), Aquaporin CD, WCH CD
计算分子量 29 kDa
观测分子量28-29, 35-38 kDa
GenBank蛋白编号NM_000486
基因名称 AQP2
Gene ID (NCBI) 359
RRIDAB_2918288
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41181
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.

实验方案

Product Specific Protocols
WB protocol for AQP2 antibody 29386-1-APDownload protocol
IHC protocol for AQP2 antibody 29386-1-APDownload protocol
IF protocol for AQP2 antibody 29386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

FASEB J

Circadian rhythm disruptions exacerbate inner ear damage in a murine endolymphatic hydrops model

Authors - Bo Pang
View Article
mouseIHC

EMBO Mol Med

Melanin-like nanoparticles slow cyst growth in ADPKD by dual inhibition of oxidative stress and CREB

Authors - Yongzhan Sun
View Article