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AQR Polyclonal antibody

AQR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24342-1-AP

Print datasheet

Synonyms

AQR, aquarius homolog (mouse), fSAP164, IBP160, KIAA0560

验证数据展示

  • WB analysis of HEK-293 using 24342-1-AP

    WB analysis of HEK-293 using 24342-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24342-1-AP

    IHC staining of human brain using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 24342-1-AP

    IHC staining of human kidney using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IP experiment of HEK-293 using 24342-1-AP

    IP experiment of HEK-293 using 24342-1-AP

    IP result of anti-AQR (IP:24342-1-AP, 4ug; Detection:24342-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24342-1-AP targets AQR in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen AQR fusion protein Ag17810 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aquarius homolog (mouse)
Synonyms AQR, aquarius homolog (mouse), fSAP164, IBP160, KIAA0560
Calculated Molecular Weight 1485 aa, 171 kDa
Observed Molecular Weight 160-171 kDa
GenBank Accession NumberBC127111
Gene Symbol AQR
Gene ID (NCBI) 9716
RRIDAB_2879501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AQR antibody 24342-1-APDownload protocol
IHC protocol for AQR antibody 24342-1-APDownload protocol
IP protocol for AQR antibody 24342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability.

Authors - Federico Teloni
View Article