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ARAP3 Polyclonal antibody

ARAP3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22578-1-AP

Print datasheet

Synonyms

ARAP3, Centaurin delta 3, CENTD3, Cnt d3, DRAG1

验证数据展示

  • IHC staining of human colon using 22578-1-AP

    IHC staining of human colon using 22578-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22578-1-AP (ARAP3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon cancer using 22578-1-AP

    IHC staining of human colon cancer using 22578-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22578-1-AP (ARAP3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22578-1-AP is ARAP3 Fusion Protein expressed in E. coli.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen ARAP3 fusion protein Ag17436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Synonyms ARAP3, Centaurin delta 3, CENTD3, Cnt d3, DRAG1
Calculated Molecular Weight 1544 aa, 170 kDa
GenBank Accession NumberBC009968
Gene Symbol ARAP3
Gene ID (NCBI) 64411
RRIDAB_2879125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins are best characterized as regulators of membrane traffic. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of ARF-dependent pathways. ARAP3 (Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3) is unique for its dual specificity GAPs (GTPase activating protein) activity for Arf6 (ADP-ribosylation factor 6) and RhoA (Ras homolog gene family member A) regulated by PtdIns(3,4,5)P3 (phosphatidylinositol (3,4,5)-trisphosphate) and a small GTPase Rap1-GTP and is involved in regulation of cell shape and adhesion.

实验方案

Product Specific Protocols
IHC protocol for ARAP3 antibody 22578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols