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ARF3 Polyclonal antibody, PBS Only

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22289-1-PBS

Print datasheet

Synonyms

ADP-ribosylation factor 3, ADP ribosylation factor 3

验证数据展示

  • WB analysis of mouse brain using 22289-1-AP (same clone as 22289-1-PBS)

    WB analysis of mouse brain using 22289-1-AP (same clone as 22289-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 22289-1-AP (same clone as 22289-1-PBS)

    IHC staining of human breast cancer using 22289-1-AP (same clone as 22289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARF3 antibody included
  • IHC staining of human breast cancer using 22289-1-AP (same clone as 22289-1-PBS)

    IHC staining of human breast cancer using 22289-1-AP (same clone as 22289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARF3 antibody included
  • IF Staining of HeLa using 22289-1-AP (same clone as 22289-1-PBS)

    IF Staining of HeLa using 22289-1-AP (same clone as 22289-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARF3 antibody (22289-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22289-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ARF3 Ready-To-Use IHC Kit. ARF3 primary antibody included.

产品信息

22289-1-PBS targets ARF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor 3
Synonyms ADP-ribosylation factor 3, ADP ribosylation factor 3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberNM_001659
Gene Symbol ARF3
Gene ID (NCBI) 377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61204
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562).The antibody is specific to ARF3. If has no cross reaction to other ARF members.