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ARF5 Polyclonal antibody

ARF5 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 15281-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IP, ELISA and More (1)

ADP-ribosylation factor 5, ADP ribosylation factor 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15281-1-AP targets ARF5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ARF5 fusion protein Ag7400 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor 5
别名 ADP-ribosylation factor 5, ADP ribosylation factor 5
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC003043
基因名称 ARF5
Gene ID (NCBI) 381
RRIDAB_2227435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP84085
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

实验方案

Product Specific Protocols
WB protocol for ARF5 antibody 15281-1-APDownload protocol
IHC protocol for ARF5 antibody 15281-1-APDownload protocol
IF protocol for ARF5 antibody 15281-1-APDownload protocol
IP protocol for ARF5 antibody 15281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Physiol Biochem

Rab11-FIP4 interacts with ARF5 to promote cancer stemness in hepatocellular carcinoma

Authors - Feifeng Song
  • KD Validated
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