验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, MCF-7 cells, pig liver tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68924-1-Ig targets ARF6 in WB, ELISA applications and shows reactivity with human, pig samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human, pig |
Immunogen | ARF6 fusion protein Ag12642 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ADP-ribosylation factor 6 |
Synonyms | EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1 |
Calculated Molecular Weight | 20 kDa |
Observed Molecular Weight | 17-20 kDa |
GenBank Accession Number | BC002952 |
Gene Symbol | ARF6 |
Gene ID (NCBI) | 382 |
RRID | AB_3670454 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P62330 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.
实验方案
Product Specific Protocols | |
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WB protocol for ARF6 antibody 68924-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |