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验证数据展示

  • WB analysis using 31684-1-AP

    WB analysis using 31684-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31684-1-AP (ARFGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31684-1-AP

    IHC staining of human ovary cancer using 31684-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31684-1-AP (ARFGEF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARFGEF1 Polyclonal antibody

ARFGEF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31684-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HEK-293T cells, HeLa cells, NIH/3T3 cells, mouse lung tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31684-1-AP targets ARFGEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ARFGEF1 fusion protein Ag35629 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
别名 p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1
计算分子量208kDa
观测分子量210 kDa
GenBank蛋白编号NM_001413184
基因名称 ARFGEF1
Gene ID (NCBI) 10565
RRIDAB_3670078
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y6D6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1), also known as BIG1 (Brefeldin A-inhibited guanine nucleotide exchange protein 1), is one of the ADP ribosylation factor (ARF) guanine nucleotide exchange factor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882).

实验方案

Product Specific Protocols
WB protocol for ARFGEF1 antibody 31684-1-APDownload protocol
IHC protocol for ARFGEF1 antibody 31684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols