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验证数据展示

  • WB analysis using 17726-1-AP

    WB analysis using 17726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17726-1-AP (Arfaptin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17726-1-AP

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of human breast cancer using 17726-1-AP

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IF Staining of HepG2 using 17726-1-AP

    IF Staining of HepG2 using 17726-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Arfaptin-1 antibody (17726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 17726-1-AP

    FC experiment of HepG2 using 17726-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Arfaptin-1 Polyclonal antibody (17726-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Arfaptin-1 Polyclonal antibody

Arfaptin-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 17726-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, Raw 264.7 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17726-1-AP targets Arfaptin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Arfaptin-1 fusion protein Ag12122 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor interacting protein 1
别名 Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521
计算分子量 373 aa, 42 kDa
观测分子量 42 kDa, 38 kDa, 22 kDa
GenBank蛋白编号BC103759
基因名称 Arfaptin-1
Gene ID (NCBI) 27236
RRIDAB_2227457
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53367
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IHC protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IF protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang
View Article