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ARFIP2 Polyclonal antibody

ARFIP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14548-1-AP

Print datasheet

Synonyms

POR1, Arfaptin-2, Arfaptin 2, ADP-ribosylation factor-interacting protein 2

验证数据展示

  • WB analysis of HeLa using 14548-1-AP

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14548-1-AP

    IF Staining of HeLa using 14548-1-AP

    Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human liver using 14548-1-AP

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ARFIP2 antibody included
  • IHC staining of human liver using 14548-1-AP

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ARFIP2 antibody included

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, human liver tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARFIP2 Ready-To-Use IHC Kit. ARFIP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14548-1-AP targets ARFIP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ARFIP2 fusion protein Ag6076 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor interacting protein 2
Synonyms POR1, Arfaptin-2, Arfaptin 2, ADP-ribosylation factor-interacting protein 2
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa, 45 kDa
GenBank Accession NumberBC000392
Gene Symbol ARFIP2
Gene ID (NCBI) 23647
RRIDAB_2242930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53365
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARFIP2 antibody 14548-1-APDownload protocol
IHC protocol for ARFIP2 antibody 14548-1-APDownload protocol
IF protocol for ARFIP2 antibody 14548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

Deciphering the immunological and prognostic features of hepatocellular carcinoma through ADP-ribosylation-related genes analysis and identify potential therapeutic target ARFIP2

Authors - Fenfen Jiang
View Article
human,mouseIHC,WB,IF

J Hepatocell Carcinoma

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway

Authors - Kaida Huang
View Article