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ARHGAP18 Polyclonal antibody

ARHGAP18 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28409-1-AP

Print datasheet

Synonyms

Rho-type GTPase-activating protein 18, Rho GTPase-activating protein 18, MacGAP

验证数据展示

  • WB analysis using 28409-1-AP

    WB analysis using 28409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28409-1-AP (ARHGAP18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28409-1-AP

    IF Staining of HeLa using 28409-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28409-1-AP (ARHGAP18 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-453s cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28409-1-AP targets ARHGAP18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ARHGAP18 fusion protein Ag29144 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 18
Synonyms Rho-type GTPase-activating protein 18, Rho GTPase-activating protein 18, MacGAP
Calculated Molecular Weight 663 aa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC107416
Gene Symbol ARHGAP18
Gene ID (NCBI) 93663
RRIDAB_2881136
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP18 antibody 28409-1-APDownload protocol
IF protocol for ARHGAP18 antibody 28409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols