验证数据展示
经过测试的应用
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18834-1-AP targets ARHGAP24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat, Canine |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 24 |
| 别名 | ARHGAP24, Filamin A associated RhoGAP, FILGAP, p73, p73RhoGAP, RC GAP72, RCGAP72, Rho type GTPase activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY SAR 88 |
| 计算分子量 | 84 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC098580 |
| 基因名称 | ARHGAP24 |
| Gene ID (NCBI) | 83478 |
| RRID | AB_10603354 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N264 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARHGAP24 antibody 18834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Curr Biol Cyclic Mechanical Loading Is Essential for Rac1-Mediated Elongation and Remodeling of the Embryonic Mitral Valve. | ||
Oncogene Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma. | ||
Oncotarget Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines. | ||
Clin Exp Pharmacol Physiol ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42. | ||
Biochem Biophys Rep Knockdown of ARHGAP24 reduces intimal hyperplasia through inhibiting the proliferation and phenotypic switching of smooth muscle cells possibly by inactivating both AKT and ERK1/2 signaling pathways |