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  • Featured Product
  • KD/KO Validated

ARHGAP25 Polyclonal antibody

ARHGAP25 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14349-1-AP

Print datasheet

Synonyms

Rho-type GTPase-activating protein 25, Rho GTPase-activating protein 25, KIAA0053

验证数据展示

  • WB analysis using 14349-1-AP

    WB analysis using 14349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14349-1-AP (ARHGAP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14349-1-AP

    IP experiment of Jurkat using 14349-1-AP

    IP result of anti-ARHGAP25 (IP:14349-1-AP, 4ug; Detection:14349-1-AP 1:500) with Jurkat cells lysate 1000ug.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of Jurkat using 14349-1-AP

    WB analysis of Jurkat using 14349-1-AP

    WB result of ARHGAP25 antibody (14349-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP25 transfected Jurkat cells.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, Ramos cells, THP-1 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14349-1-AP targets ARHGAP25 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ARHGAP25 fusion protein Ag5691 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 25
Synonyms Rho-type GTPase-activating protein 25, Rho GTPase-activating protein 25, KIAA0053
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC039591
Gene Symbol ARHGAP25
Gene ID (NCBI) 9938
RRIDAB_2059273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42331
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP25 (Rho GTPase-activating protein 25) also known as KAIA0053, belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling,cell polarity, and cell migration. ARHGAP25 has a key role in the pathomechanism of autoantibody-induced arthritis in which it regulates inflammation.

实验方案

Product Specific Protocols
WB protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IHC protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IP protocol for ARHGAP25 antibody 14349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop

Authors - Sijia Han
  • KD Validated
View Article