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验证数据展示

  • WB analysis using 14349-1-AP

    WB analysis using 14349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14349-1-AP (ARHGAP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14349-1-AP

    IP experiment of Jurkat using 14349-1-AP

    IP result of anti-ARHGAP25 (IP:14349-1-AP, 4ug; Detection:14349-1-AP 1:500) with Jurkat cells lysate 1000ug.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of Jurkat using 14349-1-AP

    WB analysis of Jurkat using 14349-1-AP

    WB result of ARHGAP25 antibody (14349-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP25 transfected Jurkat cells.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ARHGAP25 Polyclonal antibody

ARHGAP25 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14349-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Rho-type GTPase-activating protein 25, Rho GTPase-activating protein 25, KIAA0053

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Ramos cells, THP-1 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14349-1-AP targets ARHGAP25 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ARHGAP25 fusion protein Ag5691 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase activating protein 25
别名 Rho-type GTPase-activating protein 25, Rho GTPase-activating protein 25, KIAA0053
计算分子量 73 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC039591
基因名称 ARHGAP25
Gene ID (NCBI) 9938
RRIDAB_2059273
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42331
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGAP25 (Rho GTPase-activating protein 25) also known as KAIA0053, belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling,cell polarity, and cell migration. ARHGAP25 has a key role in the pathomechanism of autoantibody-induced arthritis in which it regulates inflammation.

实验方案

Product Specific Protocols
WB protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IHC protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IP protocol for ARHGAP25 antibody 14349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop

Authors - Sijia Han
  • KD Validated
View Article