验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human brain tissue, mouse thymus tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human liver tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17747-1-AP targets GRAF in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GRAF fusion protein Ag12008 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 26 |
| 别名 | ARHGAP26, OPHN1L, Oligophrenin-1-like protein, Oligophrenin 1 like protein, KIAA0621 |
| 计算分子量 | 759 aa, 86 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | BC068555 |
| 基因名称 | ARHGAP26 |
| Gene ID (NCBI) | 23092 |
| RRID | AB_2059284 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of GRAF can lead to promyelocytic leukemia, suggesting tumor suppressive activity of GRAF. GRAF was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081). It has been reported that there are three splicing mutants of GRAF, namely GRAF1a (92 kDa),GRAF1b (86 kDa) and GRAF1c (75-82 kDa), and GRAF1b and GRAF1c are the major GRAF1 isoforms in adult brain, whereas GRAF1a is abundant in neonates(PMID: 35624318, PMID: 30626696).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRAF antibody 17747-1-AP | Download protocol |
| IHC protocol for GRAF antibody 17747-1-AP | Download protocol |
| IP protocol for GRAF antibody 17747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurol Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis | ||
Brain Res Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis. | ||
Cell Death Dis CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway |
