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ARHGAP27 Polyclonal antibody

ARHGAP27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22077-1-AP

Print datasheet

Synonyms

SH3 domain-containing protein 20, Rho-type GTPase-activating protein 27, Rho GTPase-activating protein 27, PP905, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1

验证数据展示

  • WB analysis of A549 using 22077-1-AP

    WB analysis of A549 using 22077-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22077-1-AP (ARHGAP27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22077-1-AP

    IHC staining of human breast cancer using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22077-1-AP

    IHC staining of human breast cancer using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 22077-1-AP

    IF Staining of HeLa using 22077-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22077-1-AP targets ARHGAP27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ARHGAP27 fusion protein Ag17225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 27
Synonyms SH3 domain-containing protein 20, Rho-type GTPase-activating protein 27, Rho GTPase-activating protein 27, PP905, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Calculated Molecular Weight 548 aa, 62 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC101388
Gene Symbol ARHGAP27
Gene ID (NCBI) 201176
RRIDAB_2878985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZUM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IHC protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IF protocol for ARHGAP27 antibody 22077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols