• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ARHGAP4 Polyclonal antibody

ARHGAP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16697-1-AP

Print datasheet

Synonyms

RhoGAP4, Rho-GAP hematopoietic protein C1, Rho GTPase-activating protein 4, RGC1, p115

验证数据展示

  • WB analysis using 16697-1-AP

    WB analysis using 16697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16697-1-AP (ARHGAP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 16697-1-AP

    IHC staining of human lymphoma using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 16697-1-AP

    IHC staining of human lymphoma using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HL-60 using 16697-1-AP

    IP experiment of HL-60 using 16697-1-AP

    IP result of anti-ARHGAP4 (IP:16697-1-AP, 4ug; Detection:16697-1-AP 1:500) with HL-60 cells lysate 1000ug.

  • IF Staining of Jurkat using 16697-1-AP

    IF Staining of Jurkat using 16697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Jurkat using 16697-1-AP

    IF Staining of Jurkat using 16697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, human placenta tissue, mouse spleen tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman lymphoma tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16697-1-AP targets ARHGAP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ARHGAP4 fusion protein Ag10091 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho GTPase activating protein 4
Synonyms RhoGAP4, Rho-GAP hematopoietic protein C1, Rho GTPase-activating protein 4, RGC1, p115
Calculated Molecular Weight 986 aa, 109 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC052303
Gene Symbol ARHGAP4
Gene ID (NCBI) 393
RRIDAB_1850892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98171
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IHC protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IF protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IP protocol for ARHGAP4 antibody 16697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Analysis of ARHGAP4 Expression With Colorectal Cancer Clinical Characteristics and Prognosis.

Authors - Ming-Sheng Fu
View Article
humanWB,IHC

Life Sci

Prognosis, immune microenvironment, and personalized treatment prediction in Rho GTPase-activating protein 4-mutant cervical cancer: Computer strategies for precision oncology

Authors - Xiaoqin Lu
View Article
humanIHC

Cancer Biomark

Rho GTPase-activating protein 4 is upregulated in Kidney Renal Clear Cell Carcinoma and associated with poor prognosis and immune infiltration

Authors - Xuesong Xiao
View Article