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验证数据展示

  • WB analysis using 16697-1-AP

    WB analysis using 16697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16697-1-AP (ARHGAP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 16697-1-AP

    IHC staining of human lymphoma using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP4 antibody included
  • IHC staining of human lymphoma using 16697-1-AP

    IHC staining of human lymphoma using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP4 antibody included
  • IP experiment of HL-60 using 16697-1-AP

    IP experiment of HL-60 using 16697-1-AP

    IP result of anti-ARHGAP4 (IP:16697-1-AP, 4ug; Detection:16697-1-AP 1:500) with HL-60 cells lysate 1000ug.

  • IF Staining of Jurkat using 16697-1-AP

    IF Staining of Jurkat using 16697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Jurkat using 16697-1-AP

    IF Staining of Jurkat using 16697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ARHGAP4 Polyclonal antibody

ARHGAP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16697-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

RhoGAP4, Rho-GAP hematopoietic protein C1, Rho GTPase-activating protein 4, RGC1, p115

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, human placenta tissue, mouse spleen tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman lymphoma tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy ARHGAP4 Ready-To-Use IHC Kit. ARHGAP4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16697-1-AP targets ARHGAP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 ARHGAP4 fusion protein Ag10091 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho GTPase activating protein 4
别名 RhoGAP4, Rho-GAP hematopoietic protein C1, Rho GTPase-activating protein 4, RGC1, p115
计算分子量 986 aa, 109 kDa
观测分子量 115 kDa
GenBank蛋白编号BC052303
基因名称 ARHGAP4
Gene ID (NCBI) 393
RRIDAB_1850892
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP98171
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IHC protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IF protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IP protocol for ARHGAP4 antibody 16697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Analysis of ARHGAP4 Expression With Colorectal Cancer Clinical Characteristics and Prognosis.

Authors - Ming-Sheng Fu
View Article
humanWB,IHC

Life Sci

Prognosis, immune microenvironment, and personalized treatment prediction in Rho GTPase-activating protein 4-mutant cervical cancer: Computer strategies for precision oncology

Authors - Xiaoqin Lu
View Article
humanIHC

Cancer Biomark

Rho GTPase-activating protein 4 is upregulated in Kidney Renal Clear Cell Carcinoma and associated with poor prognosis and immune infiltration

Authors - Xuesong Xiao
View Article