验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy ARHGAP5 Ready-To-Use IHC Kit. ARHGAP5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
55165-1-AP targets ARHGAP5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho GTPase activating protein 5 |
| 别名 | ARHGAP5, GFI2, p190 B, RhoGAP5 |
| 计算分子量 | 172 kDa |
| 观测分子量 | 170-190 kDa |
| GenBank蛋白编号 | NM_001173 |
| 基因名称 | ARHGAP5 |
| Gene ID (NCBI) | 394 |
| RRID | AB_10858787 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13017 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGAP5, also named as RHOGAP5 and p190-B, is GTPase-activating protein for Rho family members. It may play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. ARHGAP5 can be directly phosphorylated on a single identified tyrosine residue by the activated INS and IGF-1 receptors. It also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of ARHGAP5 is shown to attenuate signal transducing activity of Rac, Rho and CDC42. The antibody is specific to ARHGAP5.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP5 antibody 55165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Med Rep Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis. | ||
Nat Metab Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis. |
