验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| CoIP | See 1 publications below |
产品信息
11363-1-AP targets ARHGEF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ARHGEF1 fusion protein Ag1905 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho guanine nucleotide exchange factor (GEF) 1 |
| 别名 | p115-RhoGEF, p115RhoGEF, P115 RHOGEF, LSC, LBCL2 |
| 计算分子量 | 912 aa, 102 kDa |
| 观测分子量 | 102-110 kDa |
| GenBank蛋白编号 | BC011726 |
| 基因名称 | ARHGEF1 |
| Gene ID (NCBI) | 9138 |
| RRID | AB_2059741 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92888 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGEF1 antibody 11363-1-AP | Download protocol |
| IHC protocol for ARHGEF1 antibody 11363-1-AP | Download protocol |
| IF protocol for ARHGEF1 antibody 11363-1-AP | Download protocol |
| IP protocol for ARHGEF1 antibody 11363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Metab Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression | ||
Mol Biol Cell The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα. | ||
Nat Commun Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis | ||
Cancer Lett Apigenin inhibits tumor angiogenesis by hindering microvesicle biogenesis via ARHGEF1 |
