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ARHGEF18 Polyclonal antibody

ARHGEF18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11243-1-AP

Print datasheet

Synonyms

p114-Rho-GEF, p114RhoGEF, P114 RhoGEF, p114 Rho GEF, KIAA0521

验证数据展示

  • WB analysis of mouse testis using 11243-1-AP

    WB analysis of mouse testis using 11243-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11243-1-AP (ARHGEF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 11243-1-AP

    IHC staining of mouse kidney using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 11243-1-AP

    IHC staining of mouse kidney using 11243-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 11243-1-AP

    IF Staining of HEK-293 using 11243-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ARHGEF18 antibody (11243-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11243-1-AP targets ARHGEF18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ARHGEF18 fusion protein Ag1749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name rho/rac guanine nucleotide exchange factor (GEF) 18
Synonyms p114-Rho-GEF, p114RhoGEF, P114 RhoGEF, p114 Rho GEF, KIAA0521
Calculated Molecular Weight 131 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC014994
Gene Symbol ARHGEF18
Gene ID (NCBI) 23370
RRIDAB_2877753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZSZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGEF18 antibody 11243-1-APDownload protocol
IHC protocol for ARHGEF18 antibody 11243-1-APDownload protocol
IF protocol for ARHGEF18 antibody 11243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols