验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, multi-cells/tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55213-1-AP targets ARHGEF4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho guanine nucleotide exchange factor (GEF) 4 |
| 别名 | ARHGEF4, ASEF, ASEF1, GEF4, KIAA1112, STM6 |
| 计算分子量 | 79 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | NM_015320 |
| 基因名称 | ARHGEF4 |
| Gene ID (NCBI) | 50649 |
| RRID | AB_2721023 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR80 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| IHC protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| IP protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration. | ||
J Neurosci Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.
| ||
iScience Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma | ||
Int J Oncol ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer. | ||
Biol Reprod Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis. | ||
Cell Adh Migr ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices.
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