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ARHGEF7 Polyclonal antibody

ARHGEF7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14092-1-AP

Print datasheet

Synonyms

KIAA0142, COOL-1, COOL1, COOL 1, Beta-Pix

验证数据展示

  • WB analysis of PC-12 using 14092-1-AP

    WB analysis of PC-12 using 14092-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14092-1-AP

    IHC staining of mouse brain using 14092-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGEF7 antibody included
  • IHC staining of mouse brain using 14092-1-AP

    IHC staining of mouse brain using 14092-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGEF7 antibody included
  • WB analysis of Transfected HEK-293 using 14092-1-AP

    WB analysis of Transfected HEK-293 using 14092-1-AP

    WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.

  • IHC staining of rat brain using 14092-1-AP

    IHC staining of rat brain using 14092-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGEF7 antibody included
  • IHC staining of rat brain using 14092-1-AP

    IHC staining of rat brain using 14092-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGEF7 antibody included
  • IP experiment of HeLa using 14092-1-AP

    IP experiment of HeLa using 14092-1-AP

    IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 14092-1-AP

    IF Staining of HeLa using 14092-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human rectal cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARHGEF7 Ready-To-Use IHC Kit. ARHGEF7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14092-1-AP targets ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ARHGEF7 fusion protein Ag5223 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho guanine nucleotide exchange factor (GEF) 7
Synonyms KIAA0142, COOL-1, COOL1, COOL 1, Beta-Pix
Calculated Molecular Weight 803 aa, 90 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC060776
Gene Symbol ARHGEF7
Gene ID (NCBI) 8874
RRIDAB_2227567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14155
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IHC protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IF protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IP protocol for ARHGEF7 antibody 14092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.

Authors - Ruth Chia
View Article
humanWB,IHC

Am J Transl Res

Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.

Authors - Tao Ma
View Article