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ARHGEF7 Polyclonal antibody

ARHGEF7 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14092-1-AP

Print datasheet

Synonyms

ARHGEF7, BETA PIX, COOL 1, COOL1, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PAK3BP, PIXB

验证数据展示

  • WB analysis of PC-12 using 14092-1-AP

    WB analysis of PC-12 using 14092-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Transfected HEK-293 using 14092-1-AP

    WB analysis of Transfected HEK-293 using 14092-1-AP

    WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negatove control and ARHGEF7 overexpressed Transfected HEK-293 cells.

  • IP experiment of HeLa using 14092-1-AP

    IP experiment of HeLa using 14092-1-AP

    IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 14092-1-AP

    IF Staining of HeLa using 14092-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14092-1-AP targets ARHGEF7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ARHGEF7 fusion protein Ag5223 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho guanine nucleotide exchange factor (GEF) 7
Synonyms ARHGEF7, BETA PIX, COOL 1, COOL1, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PAK3BP, PIXB
Calculated Molecular Weight 803 aa, 90 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC060776
Gene Symbol ARHGEF7
Gene ID (NCBI) 8874
RRIDAB_2227567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14155
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IF protocol for ARHGEF7 antibody 14092-1-APDownload protocol
IP protocol for ARHGEF7 antibody 14092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.

Authors - Ruth Chia
View Article
humanWB,IHC

Am J Transl Res

Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.

Authors - Tao Ma
View Article