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ARID1A Polyclonal antibody

ARID1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30304-1-AP

Print datasheet

Synonyms

ARID1A, B120, BAF250, BAF250a, BM029, BRG1 associated factor 250, BRG1 associated factor 250a, C1orf4, hELD, hOSA1, Osa homolog 1, OSA1, P270, SMARCF1, SWI like protein, SWI/SNF complex protein p270

验证数据展示

  • WB analysis using 30304-1-AP

    WB analysis using 30304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30304-1-AP (ARID1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 30304-1-AP

    IP experiment of HeLa using 30304-1-AP

    IP result of anti-ARID1A (IP:30304-1-AP, 4ug; Detection:30304-1-AP 1:3000) with HeLa cells lysate 1280 ug.

  • IHC staining of human endometrial cancer using 30304-1-AP

    IHC staining of human endometrial cancer using 30304-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human endometrial cancer using 30304-1-AP

    IHC staining of human endometrial cancer using 30304-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human endometrial cancer using 30304-1-AP

    IHC staining of human endometrial cancer using 30304-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human endometrial cancer using 30304-1-AP

    IHC staining of human endometrial cancer using 30304-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID1A antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARID1A Ready-To-Use IHC Kit. ARID1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30304-1-AP targets ARID1A in WB, IP, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen ARID1A fusion protein Ag30749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name AT rich interactive domain 1A (SWI-like)
Synonyms ARID1A, B120, BAF250, BAF250a, BM029, BRG1 associated factor 250, BRG1 associated factor 250a, C1orf4, hELD, hOSA1, Osa homolog 1, OSA1, P270, SMARCF1, SWI like protein, SWI/SNF complex protein p270
Calculated Molecular Weight 242 kDa
Observed Molecular Weight250-260 kDa
GenBank Accession NumberNM_006015
Gene Symbol ARID1A
Gene ID (NCBI) 8289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

实验方案

Product Specific Protocols
WB protocol for ARID1A antibody 30304-1-APDownload protocol
IHC protocol for ARID1A antibody 30304-1-APDownload protocol
IP protocol for ARID1A antibody 30304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols